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KMID : 0380020190340020080
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Korean Journal of Biotechnology and Bioengineering
2019 Volume.34 No. 2 p.80 ~ p.84
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Identification of Microorganisms Isolated from Ancient Cultural Heritage and Verification of Biomass Degrading Activities
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Park Dong-Min
Kim Saet-Byeol
Kwon Deok-Ho
Park Jae-Bum
So Myoung-Gi
Lee Byeong-Hoon
Hong Eock-Kee
Ha Suk-Jin
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Abstract
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Ancient cultural heritage is very important in historical worth which they must be preserved in. However, they are rotten and disintegrated easily by various microorganisms like fungi and bacteria because they are mostly made up of wood and paper. In order to control these microbes, we need to investigate these microbes to maintain historical worth of ancient cultural heritage. We collected several samples from surface of ancient documents and wooden artifacts. And then microorganisms were isolated from the samples and identified through sequencing of 16S ribosomal RNA or internal transcribed spacer region. As results of sequencing, two bacterial strains (E-1, O-1) were identified as Staphylococcus hominis and S. epidermidis and four fungal strains (A-1, M-1, M-2, K-1) were identified as Schizophyllum commune, Peniophora incarnata, Cephalotheca foveolata, Aspergillus niger. Among them, A-1 strain showed relatively higher cellulose and CMC degrading activities and K-1 strain showed relatively higher xylan degrading activity than other strains.
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KEYWORD
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ancient cultural heritage, microorganisms, cellulose, hemicellulose
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